Direct matrix-assisted laser desorption/ionization mass spectrometric imaging of cellulose and hemicellulose in Populus tissue.
Identifieur interne : 002F25 ( Main/Exploration ); précédent : 002F24; suivant : 002F26Direct matrix-assisted laser desorption/ionization mass spectrometric imaging of cellulose and hemicellulose in Populus tissue.
Auteurs : Kyle Ann Lunsford [États-Unis] ; Gary F. Peter ; Richard A. YostSource :
- Analytical chemistry [ 1520-6882 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Cellulose, Lignine, Populus.
- méthodes : Spectrométrie de masse MALDI.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Cellulose, Lignin.
- chemistry : Populus.
- methods : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
Imaging applied toward lignocellulosic materials requires high molecular specificity to map specific compounds within intact tissue. Although secondary ionization mass spectrometry (SIMS) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) with a single stage of MS have been used to image lignocellulosic biomass, the complexity of the plant tissue requires tandem MS, which limits the interpretation of simple MS. MALDI linear ion trap (LIT) tandem MS offers the high molecular specificity needed for lignocellulosic analyses. MALDI-LIT MS analyses of cellulose and xylan (hemicellulose) standards were performed to determine mass-to-charge ratios and fragmentation pathways for identification of these compounds in intact tissue. The MALDI-LIT-MS images of young Populus wood stem showed even distribution of both cellulose and hemicellulose ions; in contrast, the tandem MS images of cellulose and hemicellulose generated by plotting characteristic fragment ions resulted in drastically different images. This demonstrates that isobaric ions are present during MALDI-LIT-MS analyses of wood tissue and tandem MS is necessary to distinguish between isobaric species for selective imaging of carbohydrates in biomass.
DOI: 10.1021/ac2013527
PubMed: 21766865
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Yost</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21766865</idno><idno type="pmid">21766865</idno><idno type="doi">10.1021/ac2013527</idno><idno type="wicri:Area/Main/Corpus">002D38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002D38</idno><idno type="wicri:Area/Main/Curation">002D38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002D38</idno><idno type="wicri:Area/Main/Exploration">002D38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Direct matrix-assisted laser desorption/ionization mass spectrometric imaging of cellulose and hemicellulose in Populus tissue.</title><author><name sortKey="Lunsford, Kyle Ann" sort="Lunsford, Kyle Ann" uniqKey="Lunsford K" first="Kyle Ann" last="Lunsford">Kyle Ann Lunsford</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Florida, Gainesville, Florida 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Florida, Gainesville, Florida 32611</wicri:regionArea><wicri:noRegion>Florida 32611</wicri:noRegion></affiliation></author><author><name sortKey="Peter, Gary F" sort="Peter, Gary F" uniqKey="Peter G" first="Gary F" last="Peter">Gary F. Peter</name></author><author><name sortKey="Yost, Richard A" sort="Yost, Richard A" uniqKey="Yost R" first="Richard A" last="Yost">Richard A. Yost</name></author></analytic><series><title level="j">Analytical chemistry</title><idno type="eISSN">1520-6882</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cellulose (chemistry)</term><term>Lignin (chemistry)</term><term>Populus (chemistry)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellulose (composition chimique)</term><term>Lignine (composition chimique)</term><term>Populus (composition chimique)</term><term>Spectrométrie de masse MALDI (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulose</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellulose</term><term>Lignine</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Spectrométrie de masse MALDI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Imaging applied toward lignocellulosic materials requires high molecular specificity to map specific compounds within intact tissue. Although secondary ionization mass spectrometry (SIMS) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) with a single stage of MS have been used to image lignocellulosic biomass, the complexity of the plant tissue requires tandem MS, which limits the interpretation of simple MS. MALDI linear ion trap (LIT) tandem MS offers the high molecular specificity needed for lignocellulosic analyses. MALDI-LIT MS analyses of cellulose and xylan (hemicellulose) standards were performed to determine mass-to-charge ratios and fragmentation pathways for identification of these compounds in intact tissue. The MALDI-LIT-MS images of young Populus wood stem showed even distribution of both cellulose and hemicellulose ions; in contrast, the tandem MS images of cellulose and hemicellulose generated by plotting characteristic fragment ions resulted in drastically different images. This demonstrates that isobaric ions are present during MALDI-LIT-MS analyses of wood tissue and tandem MS is necessary to distinguish between isobaric species for selective imaging of carbohydrates in biomass.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21766865</PMID><DateCompleted><Year>2011</Year><Month>12</Month><Day>21</Day></DateCompleted><DateRevised><Year>2011</Year><Month>09</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6882</ISSN><JournalIssue CitedMedium="Internet"><Volume>83</Volume><Issue>17</Issue><PubDate><Year>2011</Year><Month>Sep</Month><Day>01</Day></PubDate></JournalIssue><Title>Analytical chemistry</Title><ISOAbbreviation>Anal Chem</ISOAbbreviation></Journal><ArticleTitle>Direct matrix-assisted laser desorption/ionization mass spectrometric imaging of cellulose and hemicellulose in Populus tissue.</ArticleTitle><Pagination><MedlinePgn>6722-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/ac2013527</ELocationID><Abstract><AbstractText>Imaging applied toward lignocellulosic materials requires high molecular specificity to map specific compounds within intact tissue. Although secondary ionization mass spectrometry (SIMS) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) with a single stage of MS have been used to image lignocellulosic biomass, the complexity of the plant tissue requires tandem MS, which limits the interpretation of simple MS. MALDI linear ion trap (LIT) tandem MS offers the high molecular specificity needed for lignocellulosic analyses. MALDI-LIT MS analyses of cellulose and xylan (hemicellulose) standards were performed to determine mass-to-charge ratios and fragmentation pathways for identification of these compounds in intact tissue. The MALDI-LIT-MS images of young Populus wood stem showed even distribution of both cellulose and hemicellulose ions; in contrast, the tandem MS images of cellulose and hemicellulose generated by plotting characteristic fragment ions resulted in drastically different images. This demonstrates that isobaric ions are present during MALDI-LIT-MS analyses of wood tissue and tandem MS is necessary to distinguish between isobaric species for selective imaging of carbohydrates in biomass.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lunsford</LastName><ForeName>Kyle Ann</ForeName><Initials>KA</Initials><AffiliationInfo><Affiliation>Department of Chemistry, University of Florida, Gainesville, Florida 32611, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peter</LastName><ForeName>Gary F</ForeName><Initials>GF</Initials></Author><Author ValidYN="Y"><LastName>Yost</LastName><ForeName>Richard A</ForeName><Initials>RA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>08</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Chem</MedlineTA><NlmUniqueID>0370536</NlmUniqueID><ISSNLinking>0003-2700</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>12</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21766865</ArticleId><ArticleId IdType="doi">10.1021/ac2013527</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Peter, Gary F" sort="Peter, Gary F" uniqKey="Peter G" first="Gary F" last="Peter">Gary F. Peter</name><name sortKey="Yost, Richard A" sort="Yost, Richard A" uniqKey="Yost R" first="Richard A" last="Yost">Richard A. Yost</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Lunsford, Kyle Ann" sort="Lunsford, Kyle Ann" uniqKey="Lunsford K" first="Kyle Ann" last="Lunsford">Kyle Ann Lunsford</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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